Dynorphin Works!!!!

(but Volocity still Sucks!!!)

Stitched image of  Cy 2-labeled rb anti-Dynorpnin A (1:10 K, 10 X, 11 x 6 x 2 x1). Image should contain NeuroTrace counterstain in the red channel. At this magnification, both intense labeling and high background can be seen clearly so the sections processed at 1:20k should be imaged and compared. Original file = 11800 x 6550 (or approx. 13½ x 7½ feet at screen resolution). Source G1111F S2s3.

With the idea of verifying the specificity of the label, a portion of the above section was also imaged as a z-stack at 20 X. Unfortunately the rendered projection which looked great in Volocity, exported as a blurry piece of crap. The densest label is still apparent, but the individual fibers are less distinct in the projection than at 10X. Note also slight differences in brightness of the component panels in the lower image. Both were stitch in Volocity without the 'shading correction' step. While this doesn't appear to be an issue at 10X, this shows we should use this option if it doesn't cause problems because it would be very difficult to correct in PS now that we are dealing with composite images, rather than component tiles...

Assembled image of Cy 2-labeled rb anti-Dynorpnin A (1:10 K, 20 X) stitched stack. Original image is 2 channel, 10 slice mosaic (7 x 4 x 10 x 2). In Volocity it looked fantastic but it will export TIFF as indexed color, not RGB, which is blurry & has lost the NeuroTrace FRed counterstain. This version was curved, sharpened heavily and shrunk, but that still doesn't completely hide the blur.