Cy 3 GAD Mosaic

Immuno: rb∝GAD (1:40), labeled w/ Cy3⦁SAvidin (1:760, 90 min)
Tissue: 9/21/12, G1110C
Image: 10X, 9 panels, 1024x1024, 8 bit, 1 channel = 2747 X 2745 (cropped)=38x38" @ 72 dpi
Acquistion: 555 line, 2.0%, 1.58 Airy, Spd 7, Avg 2, Gain 559, Offset 0; 7.75 sec/panel
Tile: 15% overlap; Rotation not corrected (Zen could not stitch image)
Stitching: PS Photomerge, Auto mode
Processing: In PS, Assign & Convert to profile, Flatten and Crop. Colorized with Curves; Unsharp Mask: (Split: 64% Lighten, 31% Darken); Reduce size (from 9x9 to 6x6), convert to sRGB, save as JPEG
File Name: GAD 40k 10x 9 tile sm sRGB.jpg
Original File: 120921_Cy3GADA_40k_10X_9 tile_m0(...m8).tif

Random image of Cy 3 GAD in CP & overlying CTX. Captured for simple (fast imaging, limited tiles) source images to stitch. Conclusion: Zeiss Zen is capable of stitching images with 1 or fewer tiles...