DAPI Protocol

1. Order DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride). Available from Invitrogen as either D1306 (10 mg for $85.68) or D21490 (10 mg, ultrapure for $126.63)
2. Make stock solution of 5 mg/ml by adding 2 ml DI to one 10 mg vial. Stock solution = 14.3 mM. Note that sonication may be required to get it into solution
3. Stain. Incubate tissue in 300 nM DAPI for 1 - 5 min.
4. Rinse, coverslip, mount.

Note:

• 300 nM is a 1:47667 dilution of 14. 3 mM

which means

• 1 µl DAPI stock makes 47.7 ml DAPI working

which means

•  2 ml of stock will make 95.33 liters of DAPI stain