TRIS buffers & Stocks

To make a 10X Stock for 50 mM Tris Buffered Saline (TBS), pH 7.2,

Add 1 L blue cap DI to:

• 70.2 g Tris HCl
•   6.7 g Tris Base
•    90 g NaCl

To make 1 L TBS working solution, add 100 ml TBS 7.2 Stock to 900 ml blue cap DI

Alkaline phosphate substrates require high concentraion, high pH buffers. Primarily for this reason we also occasionally make 10X 50 mM TBS, pH 8.0 and 5X 200 mM TBS, pH 8.5.

To make the 50 mM TBS, pH 8.0 10X Stock,

Add 1 L blue cap DI to:

•  44.4 g Tris HCl
•  26.5 g Tris Base
•     90 g NaCl

To make the 200 mM TBS, pH 8.5 5X Stock,

Add 1 L blue cap DI to:

•  44.4 g Tris HCl
•  87.2 g Tris Base
•     45 g NaCl

To make 1 L working solution from a 5X Stock, add 200 ml of the TBS 8.5 Stock to 800 ml bcDI

To make Tris buffers at other pH's, Molarities or for use at temperatures significantly colder ((4ºC) or hotter ( (37º) than RT, refer to  Sigma's Tris Mixing Table (pdf)