Saturday, November 3, 2012

1024 vs 2048: x,y,z preview

512 x 512 image of NT labeled w/Alexa 488. Source is 10X, 2048x2048, single channel, and 2x2x5 tiles (20 component images, 4 MB/tile and 80MB for the image). Acquisition time was 15.49 s/ tile and 5m 15s for the image

512 x 512 image of NT labeled w/Alexa 488. Source is 20X, 1024x1024, single channel, and 4x4x16 tiles (256 component images x 1 MB/Tile = 256 MB for the image). Acquisition time was 7.75 s/ tile and 35+ m the image.
Larger verision of 10X, 2048: 900 x 897 (but compression = 7)

Larger verision of 20X, 1024: 900 x 897 (but compression = 7)
Clearly more detail available in the 20X image, but differences diminish at the perspective needed to view the whole image (original images are 3634 x 3594, which is approx. 12 x 12" at print resolution, and 38 x 38" at screen resolution!)

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