Immuno Test Results-11/16/12

The immuno came out great—basically all primaries tested worked best at the highest dilution tested. That is,

• 1:40k for Paul's MCH
• 1:16k for the rabbit anti-vGlutT2
• 1:5k for Zamir's MCH
• 1:10k worked well for the NEI 

To document the results we should image at least one section of each at as close to the same atlas level as possible.

Sections to image for Paul's MCH 
1:10k = S1 on the 10, 20 40k slide
1:20k = S4 on the 10, 20 40k slide
1:40k = S1 on the 40k slide (see below)

For Zamir's MCH, an image of S3 on the 1, 2.5, 5k slide will be enough

The NEI-labeled sections are generally too rostral or caudal for a direct comparison, but there is labeling in the LHA and ACB. Time permitting, it would be nice to image S1 (ACB) and S3 (LHA, Lvl 27).

The vGlutT2 needs to be imaged as a 1 panel z-stack at 20X to clearly distinguish labeled elements. The background is very high at 1:4k so image 8 and 16k only. 


I've captured the 1st image (Paul's MCH, 1:40k) using Volocity on the 428c Zeiss, leaving only 5 or so to go...

1024 X 1002 image of Paul's MCH (1:40k), acquired as a 10X,  5 X 5 composite with Volocity, and assembled using PS and Photomerge.