In brief, all GAD67's worked great, and the 1:5k grouped worked the best (and should be used for all future GAD67 series). It could probably be diluted further, but isn't necessary (& therefore not worth the time to try it).
The VGlut2 on the other hand wasn't so clear cut. There was clear and obvious labeling in the MH at all dilutions, but it was difficult to say that differences in intensity in other brain regions were specific vGlut2 labeling. This may be due to the nature of vGlut2 labeling, and for this reason, tests should be run with the rb ∝ and/or gp ∝ VGlut2 before writing off the ms ∝ VGlut2.
Sounds good, I'll take a look at them on Friday and take some images of the GAD67 sections.
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