Wednesday, October 31, 2012

1024 vs 2048, Round 2

AVP 10X, 1024x1024: File = 512x512, 96dpi, sRGB, saved as JPEG (Q10)

AVP 10X, 2048x2048: File = 512x512, 96dpi, sRGB, saved as JPEG (Q10)
A single field of view of AVP labeling in the LHA was acquired at 10X for both, but a scan resolution of either 1024 x 1024 (top, left) or 2048 x 2048 (middle, right). Side-by-side comparison of the original, full-size images shows much greater (approx. 2X) effective resolution and detail in the 2048 x 2048 image. However, as the close ups show in the final two images, the benefit of the additional detail depends upon the output resolution and the size of the final image.




AVP 10X, 1024, cropped to 128 x 128 (= 2x original)
AVP 10X, 2048, cropped to 256 x 256 (= native resolution)







Methods: After acquisition, images were exported as TIFF, opened in PS and color converted (-->WG_RGB 1.8-->Adobe'98). A version of the 2048 image  reduced to 1024x1024 was created for comparison. Higher power comparison produced by cropping the originals to fixed size over a similar area. Posted images were additionally processed with Auto Contrast, reduced to 512x512 at 96 dpi, converted to sRGB and saved as hight quality (10) JPEG images.

2 comments:

  1. we should note the original image names, link to the other blog entry, and maybe describe the key difference between the two

    ReplyDelete
  2. should also note aperture (1 or 1.5 Airy), optical section thickness and # of slices, if any

    ReplyDelete

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